Fluorescence Lifetime Standards for Time and Frequency Domain Fluorescence Spectroscopy
نویسندگان
چکیده
منابع مشابه
Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy.
A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 degrees C were measured independently by nine laboratories using single-photon timing and multifrequency phase and modulation fluorometry instruments with lasers as excitation source. The dyes that can serve as fluorescence lifetime standards for time-domain and frequency-domain measurements are all co...
متن کاملComparison of frequency-domain and time-domain fluorescence lifetime tomography.
We compare frequency-and time-domain formulations of deep-tissue fluorescence imaging of turbid media. Simulations are used to show that time-domain fluorescence tomography, implemented via the asymptotic lifetime-based approach, offers a significantly better separability of multiple lifetime targets than a frequency-domain approach. We also demonstrate experimentally, using complex-shaped phan...
متن کاملFLCS – Fluorescence Lifetime Correlation Spectroscopy
The fusion of Time-Correlated Single Photon Counting and Fluorescence Correlation Spectroscopy, called Fluorescence Lifetime Correlation Spectroscopy (FLCS), is a method that uses picosecond time-resolved fluorescence detection for separating different FCS-contributions. The main emphasis is on the word separating. FLCS does not involve fitting of a complicated model equation to a complicated a...
متن کاملDigitally synthesized beat frequency-multiplexed fluorescence lifetime spectroscopy.
Frequency domain fluorescence lifetime imaging is a powerful technique that enables the observation of subtle changes in the molecular environment of a fluorescent probe. This technique works by measuring the phase delay between the optical emission and excitation of fluorophores as a function of modulation frequency. However, high-resolution measurements are time consuming, as the excitation m...
متن کاملFluorescence lifetime detection in turbid media using spatial frequency domain filtering of time domain measurements.
It is demonstrated that high spatial frequency filtering of time domain fluorescence signals can allow efficient detection of intrinsic fluorescence lifetimes from turbid media and the rejection of diffuse excitation leakage. The basis of this approach is the separation of diffuse fluorescence signals into diffuse and fluorescent components with distinct spatiotemporal behavior.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2007
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac062160k